Summary of the Procedure

  • Test microorganisms were grown on appropriate media.
  • Culture used for test inoculum were evaluated for sterility, washed and concentrated in sterile

    phosphate buffered saline upon harvesting.

  • The test inoculum was split into two equal parts and added to the appropriate number of

    nebulizers. Liquid culture should not exceed 20 ml per nebulizer.

  • The device was setup per protocol requirements and operated per manufacturer’s instructions.
  • The chamber was setup and the safety checklist was completed prior to test initiation.
  • Test was initiated by aerosolizing the microorganisms per the nebulizers and allowing the

    concentration to reach the required PFU/m3. Once the concentration was reached, a time zero sample is taken then the device is run for the specified contact time and an additional sample is taken for each contact time.

  • The decontamination process is run, 4 hours of UV exposure, prior to any scientists entering the testing chamber.
  • Samples are enumerated using standard dilution and plating techniques.
  • Microbial concentrations are determined after appropriate incubation times.
  • Reductions of microorganisms are calculated relative to concentration of the time zero or corresponding control run sample as applicable

    See full study

About the author : Morten

Join our mailing list today

Insider offers & flash sales in your inbox every week.

Thank you for your message. It has been sent.
There was an error trying to send your message. Please try again later.

Curabitur non nulla sit amet nisl tempus convallis quis ac lectus dolor sit amet, consectetur adipiscing elit sed porttitor lectus.