Summary of the Procedure
- Test microorganisms were grown on appropriate media.
- Culture used for test inoculum were evaluated for sterility, washed and concentrated in sterile
phosphate buffered saline upon harvesting.
- The test inoculum was split into two equal parts and added to the appropriate number of
nebulizers. Liquid culture should not exceed 20 ml per nebulizer.
- The device was setup per protocol requirements and operated per manufacturer’s instructions.
- The chamber was setup and the safety checklist was completed prior to test initiation.
- Test was initiated by aerosolizing the microorganisms per the nebulizers and allowing the
concentration to reach the required PFU/m3. Once the concentration was reached, a time zero sample is taken then the device is run for the specified contact time and an additional sample is taken for each contact time.
- The decontamination process is run, 4 hours of UV exposure, prior to any scientists entering the testing chamber.
- Samples are enumerated using standard dilution and plating techniques.
- Microbial concentrations are determined after appropriate incubation times.
- Reductions of microorganisms are calculated relative to concentration of the time zero or corresponding control run sample as applicable